Legumes were planted in a community garden and in the laboratory with potting soil, vermiculite, and vermiculite with inoculated rhizobium. At termination, the roots were searched for nodules. The material used were: 12 pots, 2 bags of vermiculite, a bag of potting soil, 36 snow pea seeds, Bacteria (Rhizobium leguminosarum) from the Carolina Biological Supply Company (Catalog ER-15-5270, non-pathogenic), nutrient broth, an inoculating loop, and a Bunsen burner.
There was an average of 12 nodules in community garden plants, 3 for potting soil, and none in both the vermiculite with and without bacteria.
The presence of rhizobium bacteria is a factor affecting nodule formation. The results from my field observation and from the positive control group indicate that the factors affecting the formation of nodules, is not just the presence of the Rhizobium bacteria, but also the time factor, temperature factor, as well as the nutrient factor and others.
The project was to find what factors such as time, nutrients, and moisture, affect nodule formation in legumes.
Science Fair Project done By Raman V. Nelakanti