Science Fair Projects

Effects of Temperature on Bacterial Pigmentation


The objective: I really enjoyed my project this year. In my project, I tested how temperature affected the pigmentation of four different bacteria. From my research, I knew that the bacterium Serratia marcescens alters its color at different temperatures, and I wanted to explore this. I also wanted to see if this applied to other pigmented bacteria.


My project consisted of qualitative tests. First, I gathered my types of bacteria. I used Serratia marcescens, Rhodospirillum rubrum, Micrococcus luteus, and Sarcina aurantiaca. Next, I made agar from a powder. I then sterilized it and poured it into plates. After that, I made nutrient broth for the bacteria to grow in. I also sterilized this. I then inoculated the broth with the bacteria and let it grow for a few days. Next, I began my first set of tests. Using a pipette, I put each of the four bacteria onto two different plates. I then spread the bacteria with a bent glass tube. I let these incubate for two days and then observed them. I recorded the colors and drew images of the plates. I repeated this process eight times, doing tests at 25, 28, 31, 34, 35, 36, 37, 40, and 43 degrees Celsius.


Analyzing my results, it appears that Serratia marcescens is a pretty special bacterium. All the other pigmented bacteria I tested held the same coloration, whereas Serratia marcescens lost its red pigmentation at 37 degrees Celsius.


The pigment in Serratia marcescens is called prodigiosin. Once the growth temperature of Serratia marcescens is raised to 37 degrees, the pigment stops being produced. It is believed that an enzyme used in the production of prodigiosin is affected by the temperature so that the pigment is no longer made. As you can see, my project came up with some pretty interesting results

This experiment is about how temperature affects the pigmentation of Serratia marcescens, Rhodospirillum rubrum, Sarcina aurantiaca, and Micrococcus luteus.

Science Fair Project done By Caitlin R.S. Merrill


Related Projects : Human Battery, Measuring Human Horsepower, Does a Mouse Rely More on His Spatial Memory or Vision, Conserving Agricultural Irrigation Water, Effects of Ocean Water as an Irrigation Supplement , How Does Music Affect Plant Growth, Factors Affecting Nodule Formations in Legumes , What Do Plants Need Most , Compost or Fertilizer, Effect of Sugar on Bean Plant Growth , Does the Color of Light Affect Plant Growth , Is Spinach Made in the Shade , The Speed of Sprouting Seeds , Investigating Seed Tolerance for Freezing Temperatures, Fun with Phosphorus


<<Back To Topics Page........................................................................................>> Next Topic



Copyright © 2013 through 2015