This novel, "digitally-enhanced" TLC (DE TLC) is very easy to use. A fluorescent TLC plate is illuminated with UV light and a picture of the plate is taken with a digital camera. Then, on a computer, using either TLC Analyzer, the public domain software I wrote, or common photo-editing software, one can quickly produce multi-spectral scans, densitograms, and calibration curves--output previously available only from more expensive equipment or complex procedures.
With high linearity (R^2 ~ 0.97 - 0.99), good repeatability RSD < 5%), and detection limits approaching those of HPTLC, DE TLC produces surprisingly good results for such inexpensive equipment.
Digitally-Enhanced TLC is a valuable tool that can be added to every chemist's TLC toolbox. Since this technique is much less expensive than other quantitative chromatographic methods, DE TLC is ideal for high school and college labs.
This project developed an inexpensive technique using digital photography that is an alternative to a $30,000 piece of equipment for chemical analysis.
Science Fair Project done By Amber I. Hess