Nine 2-liter soda bottles were scratched to three levels of opaqueness. Six bottles were left unscratched (3 were controls). An equal amount and concentration of e coli was added to each bottle. The bottles were sealed and placed horizontally in the sun (the control were left in darkness). Samples from each bottle were inoculated onto agar plates, incubated at 37 degrees for 24 hours, and then compared to the controls.
All levels of scratching (light, medium, and heavy) allowed for the presence of e coli after sun exposure, with the Level 3 (scratchiest bottles) having the highest average number of escherichia coli colonies. Only the clear bottles showed no evidence of e coli growth. The e coli growth was highest for the controls.
There is a direct relationship between the scratchiness of the sun-exposed clear plastic bottles and the presence of e coli. Based on my results, only unscratched clear bottles should be used for SODIS. Further research could possibly reveal whether longer UV exposure might minimize the affects of the bottle opaqueness, especially for the lightly scratched bottles.
This experiment tests one of the variables present when using the SODIS method to disinfect drinking water.
Science Fair Project done By Kelli M. Muirheid