10 samples of steers on grain, steers on hay, goats on grain, and goats on hay,
40 test tubes with rubber corks, Incubator, nutrient agar plates, rubber gloves Electronic gram scale, 40 250ML jars
1.Take all samples of manure. Remember the samples aren#t taken from one animal, but rather a pool of
different animals from that diet and species.
2.Dilute the manures 1- 100 (manure to water).
3.Put 5 drops of the dilutions on the plates and spread it with the spreader.
4.Seal the plates with parafilm.
5.Put the plates in the incubator for 48 hours.
6.At the end of 48 hours take the plates out of the incubator and count the number of colonies growing on the plates by doting the colonies with a sharpie.
The results show that the steers on grain ranked the highest in microorganism growth. The goats had the lowest counts and the goats on grain ranked the absolute lowest.
In conclusion, the steers that were fed grain had more microorganisms growing than the other samples. The goats on grain had the least amount growing. This is surprisingly just what I expected. The steers have the largest digestive systems so it gives the manure more time to pick up any microorganisms. I also thought that the grain diets would have a higher count because there are more nutrients. On this I was partly correct. This was true for the steers, but not for the goats. This may have been because the ten samples I took from the goats on grain were young so they might not have had fully developed rumens. Therefore, this may be why the goats on grain had the lowest count.
This experiment is to find out if different diets of the ruminant species affect the growth of microorganisms.
Science Fair Project done By Alex J. Thompson